抗RANTES抗体(Anti-RANTES, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL5/RANTES
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human CCL5/RANTES and recombinant cotton rat CCL5/RANTES is observed and approximately 10% cross-reactivity with recombinant mouse CCL3/MIP-1 alpha is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL5/RANTES (Catalog # 478-MR)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.07-0.3 µg/mL in the presence of 0.05 µg/mL Recombinant Mouse CCL5/RANTES.

Neutralization
	Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478‑MR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND50 is typically 0.07-0.3 µg/mL.
Immunocytochemistry
	CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

目次に戻る

Related Product & Information

Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Background	CCL5/RANTES
background_content	Background: CCL5/RANTES CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

目次に戻る

Citations

1. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation
   Authors: YT Chu, SW Wan, YC Chang, CK Lee, BA Wu-Hsieh, R Anderson, YS Lin
   Lab. Invest, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
   Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
   Sci Rep, 2017;7(0):46514.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Valproate hampers podocyte acquisition of immune phenotypes via intercepting the GSK3? facilitated NFkB activation
   Authors: P Wang, S Zhou, Y Ge, M Lu, Z Liu, R Gong
   Oncotarget, 2017;8(51):88332-88344.
   Species: Mouse
   Sample Type: Tissue Homogenates
   


4. Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy
   Authors: N Suffee, C Le Visage, H Hlawaty, R Aid-Launai, V Vanneaux, J Larghero, O Haddad, O Oudar, N Charnaux, A Sutton
   Sci Rep, 2017;7(1):13294.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


5. Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease
   J Biol Chem, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


6. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells.
   Authors: Herz J, Johnson K, McGavern D
   J Exp Med, 2015;212(8):1153-69.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
   Authors: Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J
   Endocrinology, 2015;156(5):1782-93.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Astrocytic TGF-beta signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection.
   Authors: Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, Koshy A, Buckwalter M
   J Immunol, 2014;193(1):139-49.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression.
   Authors: Iida, Yasunori, Xu, Baohui, Xuan, Haojun, Glover, Keith J, Tanaka, Hiroki, Hu, Xiaolei, Fujimura, Naoki, Wang, Wei, Schultz, Joshua R, Turner, Court R, Dalman, Ronald L
   Arterioscler Thromb Vasc Biol, 2013;33(4):718-26.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis.
    Authors: Mishra, Pramod K, Teale, Judy M
    PLoS Negl Trop Dis, 2013;7(3):e2099.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded

目次に戻る