抗Fractalkine抗体(Anti-Fractalkine, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CX3CL1/FractalkineLeu22-Lys105Accession # O35188
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Fractalkine in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Fractalkine and recombinant rat Fractalkine, 20% cross-reactivity with rhMCP-1, and 10% cross-reactivity with rhEotaxin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CX3CL1/Fractalkine Full Length
Neutralization	Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105.

Neutralization

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND50 is typically 0.3-1.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
Background	CX3CL1/Fractalkine
background_content	Background: CX3CL1/Fractalkine CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

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Citations

1. Absence of CX3CR1 impairs the internalization of Tau by microglia
   Authors: M Bolós, M Llorens-Ma, JR Perea, J Jurado-Arj, A Rábano, F Hernández, J Avila
   Mol Neurodegener, 2017;12(1):59.
   


2. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow
   Authors: Yoh Goto
   Stem Cells, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
   Authors: Bryan Simons
   J. Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


4. Interaction between CX3CL1 and CX3CR1 regulates vasculitis induced by immune complex deposition.
   Authors: Morimura S, Sugaya M, Sato S
   Am J Pathol, 2013;182(5):1640-7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


5. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function.
   Authors: Ishida Y, Gao JL, Murphy PM
   J. Immunol., 2008;180(1):569-79.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
   Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
   J. Immunol., 2008;181(6):4208-18.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
   Authors: Ren T, Chen Q, Tian Z, Wei H
   Biochem. Biophys. Res. Commun., 2007;364(4):978-84.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
   Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
   J. Leukoc. Biol., 2007;81(1):176-85.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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