抗GRO抗体(Anti-GRO, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1Arg20-Lys96Accession # P12850
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed, 10% cross-reactivity with recombinant mouse (rm) MIP-2 and recombinant human (rh) IL‑8 is observed, and less than 5% cross-reactivity with rhGRO alpha, rhGRO beta, rmCXCL3, rrCINC-2 alpha, and rrCINC-2 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Neutralization	Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.

Neutralization

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND50 is typically 0.3‑1.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	2919 (Human); 14825 (Mouse); 81503 (Rat)
Background	CXCL1/GRO alpha/KC/CINC-1
background_content	Background: CXCL1/GRO alpha/KC/CINC-1 KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM.

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Citations

1. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
   Sci Rep, 2016;6(0):37695.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


2. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
   Sci Rep, 2016;6(0):37695.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination.
   Authors: Marro B, Grist J, Lane T
   J Immunol, 2016;196(4):1855-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fluorescence


4. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
   Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
   J Biol Chem, 2015;290(4):2053-68.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
   Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
   Int J Cancer, 2014;134(6):1346-58.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC- Frozen


6. Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
   Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
   Mucosal Immunol, 2012;5(5):524-34.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
   Authors: Li Z, Burns AR, Miller SB, Smith CW
   FASEB J., 2011;25(8):2659-68.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation.
   Authors: Tilahun AY, Theuer JE, Patel R
   Mol. Ther., 2010;18(6):1143-54.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
   Authors: Summers SA, van der Veen BS, O'Sullivan KM
   Kidney Int., 2010;78(12):1263-74.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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