抗IL-17抗体(Anti-IL-17, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL‑17Thr22-Ala158Accession # Q62386
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse and rat IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑17 (Catalog # 421-ML)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below
Neutralization	Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Intracellular Staining by Flow Cytometry
	Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA then stained with Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Neutralization
	IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Anti­body (Catalog # AF-421-NA). The ND50 is typically0.05-0.25 µg/mL.

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Related Product & Information

Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Background	IL-17/IL-17A
background_content	Background: IL-17/IL-17A Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse, and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

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Citations

1. IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment.
   Authors: Benevides L, da Fonseca D, Donate P, Tiezzi D, De Carvalho D, de Andrade J, Martins G, Silva J
   Cancer Res, 2015;75(18):3788-99.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


2. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
   Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
   J. Immunol., 2011;186(6):3462-71.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
   Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
   Am. J. Pathol., 2011;178(3):1106-16.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
   Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
   Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis.
   Authors: Otani K, Watanabe T, Tanigawa T, Okazaki H, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Arakawa T
   Biochem. Biophys. Res. Commun., 2009;382(2):252-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


6. Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
   Authors: Yuan X, Ansari MJ, D'Addio F, Paez-Cortez J, Schmitt I, Donnarumma M, Boenisch O, Zhao X, Popoola J, Clarkson MR, Yagita H, Akiba H, Freeman GJ, Iacomini J, Turka LA, Glimcher LH, Sayegh MH
   Proc. Natl. Acad. Sci. U.S.A., 2009;106(26):10734-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts.
   Authors: Venkatachalam K, Mummidi S, Cortez DM, Prabhu SD, Valente AJ, Chandrasekar B
   Am. J. Physiol. Heart Circ. Physiol., 2008;294(5):H2078-87.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


8. Tumor-specific Th17-polarized cells eradicate large established melanoma.
   Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
   Blood, 2008;112(2):362-73.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
   Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
   J. Immunol., 2007;178(3):1341-8.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
    Authors: Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A
    J. Immunol., 2003;170(4):2106-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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