抗PARC抗体(Anti-PARC, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL18/PARCAla21-Ala89Accession # P55774
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL18/PARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant mouse MIP-1 alpha is observed and less than 10% cross-reactivity with rhMPIF‑1, recombinant viral (rv) MIP‑I, rvMIP‑II, rhHCC-1, and rhMIP-1δ is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CCL18/PARC (Catalog # 394-PA)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB), and LPS, fixed with paraformaldehyde and permeabilized with saponin

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Related Product & Information

Background

CCL18/PARC

background_content

Background: CCL18/PARC CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

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Citations

1. High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis.
   Authors: Momohara S, Okamoto H, Iwamoto T, Mizumura T, Ikari K, Kawaguchi Y, Takeuchi M, Kamatani N, Tomatsu T
   J. Rheumatol., 2007;34(2):266-71.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18.
   Authors: Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP
   Arthritis Rheum., 2006;54(8):2643-55.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


3. Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
   Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
   J. Allergy Clin. Immunol., 2006;118(2):354-60.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells.
   Authors: Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM
   J. Immunol., 2005;174(3):1723-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.
   Authors: Pivarcsi A, Gombert M, Dieu-Nosjean MC, Kubitza R, Meller S, Rieker J, Muller A, Da Cunha L, Haahtela A, Sonkoly E, Fridman WH, Alenius H, Kemeny L, Ruzicka T, Zlotnik A
   J. Immunol., 2004;173(9):5810-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC

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