抗MIG抗体(Anti-MIG, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL9/MIGThr23-Thr125Accession # Q07325
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL9/MIG in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CXCL9 (non-reducing conditions) is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CXCL9/MIG (Catalog # 392-MG)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 1 μg/mL Recombinant Human CXCL9/MIG.

Neutralization
	Chemotaxis Induced by CXCL9/MIG and Neutral­ization by Human CXCL9/MIG Antibody. Recombinant Human CXCL9/MIG (Catalog # 392‑MG) chemo­attracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL9/MIG (1 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF392). The ND50 is typically 3‑12 µg/mL.
Immunocytochemistry
	CXCL9/MIG in THP‑1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line stimulated with IFN-gamma using Goat Anti-Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF392) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

目次に戻る

Related Product & Information

Entrez Gene IDs	4283 (Human); 17329 (Mouse); 246759 (Rat)
Background	CXCL9/MIG
background_content	Background: CXCL9/MIG CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

目次に戻る

Citations

1. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
   Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
   BMC Cancer, 2014;14(0):118.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fresh


3. Expression and regulation of chemokines in murine and human type 1 diabetes.
   Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
   Diabetes, 2012;61(2):436-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Myocarditis in CD8-depleted SIV-infected rhesus macaques after short-term dual therapy with nucleoside and nucleotide reverse transcriptase inhibitors.
   Authors: Annamalai L, Westmoreland SV, Domingues HG
   PLoS ONE, 2010;5(12):e14429.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma.
   Authors: Birgersdotter A, Baumforth KR, Porwit A, Sjoberg J, Wei W, Bjorkholm M, Murray PG, Ernberg I
   Br. J. Cancer, 2009;101(8):1393-401.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


6. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
   Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
   J. Immunol., 2009;182(6):3919-27.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
   Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
   Arthritis Rheum., 2005;52(5):1504-16.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Fresh/Frozen


8. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
   Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
   Int. J. Oncol., 2004;25(3):605-13.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
   Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
   J. Immunol., 2002;169(11):6515-21.
   Species: Human
   Sample Type: BALF
   Application: ELISA Development

目次に戻る