抗BMP Receptor 1A抗体(Anti-BMP Receptor 1A, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human BMPR-IA/AKL-3Gln24-Arg152Accession # P36894
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human BMPR-IA in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse BMPR-IA is observed and less than 5% cross-reactivity with recombinant human BMPR‑IB  is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	PC‑3 human prostate cancer cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot

Detection of Human BMPR‑IA/ALK‑3 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human BMPR‑IA/ALK‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BMPR‑IA/ALK‑3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Related Product & Information

Background

BMPR-IA/ALK-3

background_content

Background: BMPR-IA/ALK-3 Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

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Citations

1. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis.
   Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A
   PLoS ONE, 2015;10(6):e0131453.
   


2. Optimal effector functions in human natural killer cells rely upon autocrine bone morphogenetic protein signaling.
   Authors: Robson N, Hidalgo L, McAlpine T, Wei H, Martinez V, Entrena A, Melen G, MacDonald A, Phythian-Adams A, Sacedon R, Maraskovsky E, Cebon J, Ramirez M, Vicente A, Varas A
   Cancer Res, 2014;74(18):5019-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity.
   Authors: Cao Y, Slaney C, Bidwell B, Parker B, Johnstone C, Rautela J, Eckhardt B, Anderson R
   Cancer Res, 2014;74(18):5091-102.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
   Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
   Cell Signal, 2013;25(4):717-28.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells.
   Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA
   Cancer Cell, 2008;13(1):69-80.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
   Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
   J. Biol. Chem., 2007;282(32):23337-47.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
   Authors: Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O
   Stem Cells, 2007;25(4):939-49.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


8. Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
   Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
   J. Pathol., 2006;209(2):190-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
   Authors: Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK
   Brain Res., 2006;1088(1):49-56.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP

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