抗SDF-1抗体(Anti-SDF-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL12/SDF‑1 alpha Lys22-Lys89Accession # Q6ICW0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human/Feline CXCL12/SDF-1 beta aa 19-93 (Catalog # 2716-SD) Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS) Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Neutralization

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-HumanCXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Background	CXCL12/SDF-1
background_content	Background: CXCL12/SDF-1 CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

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Citations

1. Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines.
   Authors: Hanes M, Salanga C, Chowdry A, Comerford I, McColl S, Kufareva I, Handel T
   J Biol Chem, 2015;290(37):22385-97.
   Species: Human
   Sample Type: Virus
   Application: ELISA Capture


2. COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
   Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F
   BMC Cancer, 2014;14(0):407.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
   Authors: Ruiz E, Oeztuerk-Winder F, Ventura J
   Nat Commun, 2014;5(0):3175.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
   Authors: Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M
   J Immunol, 2014;192(5):2133-42.
   


5. Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells.
   Authors: Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit M, Blanchet X, von Hundelshausen P, Weber C, Forssmann W, Henschler R
   Stem Cells Dev, 2014;23(16):1959-74.
   Species: Human
   Sample Type: Plasma
   Application: Functional Assay


6. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
   Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
   Eur. J. Immunol., 2008;38(11):3050-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.
   Authors: Brzoska E, Grabowska I, Hoser G, Streminska W, Wasilewska D, Machaj EK, Pojda Z, Moraczewski J, Kawiak J
   Exp. Hematol., 2006;34(9):1262-70.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. Functional gene expression analysis of clonal plasma cells identifies a unique molecular profile for light chain amyloidosis.
   Authors: Abraham RS, Ballman KV, Dispenzieri A, Grill DE, Manske MK, Price-Troska TL, Paz NG, Gertz MA, Fonseca R
   Blood, 2005;105(2):794-803.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
   Authors: Carman CV, Springer TA
   J. Cell Biol., 2004;167(2):377-88.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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