抗HGF抗体(Anti-HGF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)Gln32-Ser728Accession # P14210
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human HGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human HGF (Catalog # 294-HG)
Neutralization	Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883. The Neutralization Dose (ND50) is typically <2 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.

Neutralization

IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HGN) induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA). The ND50 is typically 2 μg/mL.

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Related Product & Information

Long Name	Hepatocyte Growth Factor
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 403441 (Canine)
Background	HGF
background_content	Background: HGF HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

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Citations

1. Hepatocyte growth factor produced in lung fibroblasts enhances non-small cell lung cancer cell survival and tumor progression
   Authors: N Kanaji, M Yokohira, Y Nakano-Nar, N Watanabe, K Imaida, N Kadowaki, S Bandoh
   Respir. Res., 2017;18(1):118.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. c-Met-mediated endothelial plasticity drives aberrant vascularization and chemoresistance in glioblastoma
   Authors: M Huang, T Liu, P Ma, RA Mitteer, Z Zhang, HJ Kim, E Yeo, D Zhang, P Cai, C Li, L Zhang, B Zhao, L Roccogrand, DM O'Rourke, N Dahmane, Y Gong, C Koumenis, Y Fan
   J Clin Invest, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
   Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
   Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9.
   Species: Rat
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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