抗GROα抗体(Anti-GROα, Human, Goat-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：26879
GROαに対する抗体(Anti-GROα, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-GROα, Human, Goat-Poly |
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Anti-Human CXCL1/GRO alpha Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-GROα, Human, Goat-Poly
文献数： 6
- 商品コード：AF275
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human CXCL1/GRO alpha Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF275-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 Genbank No： 2919 Protein Accession No： P09341 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1 (R&D Systems, Catalog # 275-GR)Ala35-Asn107Accession # P09341 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant human (rh) GRO beta and rhGRO gamma is observed, less than 25% cross-reactivity with recombinant rat (rr) CINC-1 is observed and less than 15% cross-reactivity with recombinant mouse (rm) KC is observed. In Western blots, no cross-reactivity with rhNAP-2 or rhPF-4 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.01 µg/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1. | |
| Western Blot | |
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 | Detection of Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR), Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC), Recombinant Rat CXCL1/GRO alpha /KC/CINC‑1 (Catalog #515-CN), Recombinant Human CXCL2/GRO beta /MIP‑2/CINC‑3 aa 35-107 (Catalog # 276-GB), Recombinant Human CXCL3/GRO gamma /CINC‑2/DCIP‑1 (Catalog # 277-GG), Recombinant HumanCXCL7/NAP‑2 (Catalog # 393-NP), and Recombinant Human CXCL4/PF4 (Catalog # 795-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CXCL1/GRO alpha /KC/CINC‑1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
| Neutralization | |
 | Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody. Recombinant Human CXCL1/GRO alpha (Catalog #275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GRO alpha (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275). The ND50 is typically0.03-0.15 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 2919 (Human); 14825 (Mouse); 81503 (Rat) |
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| Background | CXCL1/GRO alpha/KC/CINC-1 |
| background_content | Background: CXCL1/GRO alpha/KC/CINC-1 The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
J. Immunol., 2002;169(11):6515-21.
Species: Human
Sample Type: BALF
Application: ELISA Development
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