抗IL-5 Receptor α抗体(Anti-IL-5 Receptor α, Human, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：26861
IL-5 Receptor αに対する抗体(Anti-IL-5 Receptor α, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年06月03日 12時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
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Anti-IL-5 Receptor α, Human, Goat-Poly |
|
4 | |||
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Anti-Human IL-5 R alpha Affinity Purified Polyclonal Ab |
|
0 | |||
[在庫・価格 ：2025年06月03日 12時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-5 Receptor α, Human, Goat-Poly
文献数： 4
- 商品コード：AF-253-NA
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：ご照会下さい ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human IL-5 R alpha Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF-253-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5 R alpha /CD125 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human IL-5 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL‑1 RII, rhIL-2 R beta, rhIL-2 R gamma, rhIL-3 R is observed and less than 1% cross-reactivity with rhIL‑1 RI, rhIL‑4 R, rhIL‑6 R, rhIL‑7 R, rhIL‑9 R, and rhIL‑10 R is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human IL‑5 R alpha /CD125 (Catalog # 253-5R) |
| Flow Cytometry | 2.5 µg/106 cells | Human blood‑derived granulocytes |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Neutralization | Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑5. | |
| Neutralization | |
|---|---|
 | Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 R alpha /CD125 Antibody. Recombinant Human IL‑5 (Catalog # 205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 R alpha /CD125 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-253-NA). The ND50 is typically 1-3 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 5 Receptor alpha |
|---|---|
| Entrez Gene IDs | 3568 (Human); 16192 (Mouse) |
| Background | IL-5 R alpha/CD125 |
| background_content | Background: IL-5 R alpha/CD125 Interleukin 5, produced primarily by activated T cells and mast cells, has diverse biological effects on a variety of cell types. Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro. Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. The multiple effects of IL-5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. As is the case with many other cytokines, the functional high-affinity receptor for IL-5 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. In the case of IL-5, the beta subunit is shared with the high affinity receptor complexes for IL-3 and GM-CSF. The beta chain does not bind any of the cytokines in question but is indispensable for the cytokine-mediated signaling. cDNA clones for the alpha chain (IL-5 R alpha ) of both the mouse and human high affinity IL-5 receptor complexes have been isolated. Human and mouse IL-5 R alpha are both members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones that arise from alternative splicing and that encode soluble secreted forms of IL-5 R alpha have been isolated from mouse as well as human cells. A naturally-occurring soluble form of the IL-5 R alpha has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia (BCL1). A recombinant human IL-5 soluble receptor alpha has been shown to bind the human IL-5 dimer in a 1:1 ratio and acts as a human IL-5 antagonist. This molecule inhibits the proliferation of IL-5-dependent cell lines and blocks human umbilical cord blood eosinophil differentiation. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Three lysine residues in the common beta chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling.
Authors: Lei JT, Mazumdar T, Martinez-Moczygemba M
J. Biol. Chem., 2011;286(46):40091-103.
Species: Human
Sample Type: Cell Lysates
Application: WB - Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
Authors: Lei JT, Martinez-Moczygemba M
J. Leukoc. Biol., 2008;84(2):499-509.
Species: Human
Sample Type: Cell Lysates
Application: WB - T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002;110(4):624-33.
Species: Human
Sample Type: Whole Cells
Application: Neut
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