抗BDNF抗体(Anti-BDNF, Human, Chicken-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNFHis129-Arg247Accession # P23560
Source	Polyclonal Chicken IgY
Purification	Antigen Affinity-purified from egg yolks
Specificity	Detects human BDNF in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	NTera‑2 human testicular embryonic carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Immunohistochemistry
	BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) at 15 µg/mL overnight at 4 °C. Tissue was stained using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

BDNF

background_content

Background: BDNF Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

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Citations

1. Acid fibroblast growth factor and peripheral nerve grafts regulate Th2 cytokine expression, macrophage activation, polyamine synthesis, and neurotrophin expression in transected rat spinal cords.
   Authors: Kuo HS, Tsai MJ, Huang MC
   J. Neurosci., 2011;31(11):4137-47.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


2. Brain-derived neurotrophic factor mediates non-cell-autonomous regulation of sensory neuron position and identity.
   Authors: Wright MA, Ribera AB,
   J. Neurosci., 2010;30(43):14513-21.
   Species: Zebrafish
   Sample Type: In Vivo
   Application: In vivo


3. PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex.
   Authors: Ohtsuka M, Fukumitsu H, Furukawa S
   Biochem. Biophys. Res. Commun., 2008;369(4):1144-9.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
   Authors: Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
   Eur. J. Neurosci., 2008;27(8):2089-102.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
   Authors: Hu B, Nikolakopoulou AM, Cohen-Cory S
   Development, 2005;132(19):4285-98.
   Species: Xenopus
   Sample Type: In Vivo
   Application: In vivo

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