抗CXCL8/IL-8抗体(Anti-IL-8, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-8/CXCL8Ser28-Ser99Accession # P10145
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, and rhGRO gamma is observed, and less than 1% cross-reactivity with recombinant feline IL-8/CXCL8, recombinant porcine IL-8/CXCL8, and recombinant canine IL-8/CXCL8 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
Neutralization	Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Neutralization

Chemotaxis Induced byIL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND50 is typically 5-20 µg/mL.

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Related Product & Information

Long Name	Interleukin 8
Entrez Gene IDs	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Background	IL-8/CXCL8
background_content	Background: IL-8/CXCL8 CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

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Citations

1. Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis
   Authors: A Angelini, Y Miyabe, D Newsted, BH Kwan, C Miyabe, RL Kelly, MN Jamy, AD Luster, KD Wittrup
   Nat Commun, 2018;9(1):1461.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Evidence for chemokine synergy during neutrophil migration in ARDS
   Thorax, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Salmonella enteritidis Effector AvrA Stabilizes Intestinal Tight Junctions via the JNK Pathway
   Authors: Zhijie Lin
   J. Biol. Chem, 2016;291(52):26837-26849.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


4. Expression and regulation of chemokines in murine and human type 1 diabetes.
   Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
   Diabetes, 2012;61(2):436-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Ctrl


5. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
   Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
   Nat. Med., 2011;17(11):1498-503.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
   Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
   J. Immunol., 2007;179(5):3332-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
   Authors: Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ
   Exp. Lung Res., 2004;30(1):43-57.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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