抗CD40 Ligand抗体(Anti-CD40 Ligand, Mouse, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26789

CD40 Ligandに対する抗体(Anti-CD40 Ligand, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2025年07月07日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-CD40 Ligand, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
サンドイッチELISAで,#BAF1163抗体(RSD社)と共に用いる際,捕捉用抗体として利用できる。
別名:CD154
Genbank No: 959
Protein Accession No: P27548
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事
Anti-Mouse CD40 Ligand/TNFSF5 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:CD154
Genbank No: 959
Protein Accession No: P27548
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事

[在庫・価格 :2025年07月07日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CD40 Ligand, Mouse, Goat-Poly

文献数: 8

説明文 サンドイッチELISAで,#BAF1163抗体(RSD社)と共に用いる際,捕捉用抗体として利用できる。
別名:CD154
Genbank No: 959
Protein Accession No: P27548
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事

Anti-Mouse CD40 Ligand/TNFSF5 Affinity Purified Polyclonal Ab

文献数: 1

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:CD154
Genbank No: 959
Protein Accession No: P27548
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Mouse 適用 IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事



目次に戻る

Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD40 Ligand/TNFSF5Glu61-Leu260Accession # P27548
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects mouse CD40 Ligand/TNFSF5 in Western blots.


目次に戻る

Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 1163-CL)
Immunocytochemistry5-15 µg/mLSee below
NeutralizationMeasured by its ability to neutralize CD40 Ligand/TNFSF5-induced proliferation in mouse splenic B cells. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse CD40 Ligand/TNFSF5.


Immunocytochemistry
CD40 Ligand/TNFSF5 in Mouse Splenocytes.
CD40 Ligand/TNFSF5 was detected in immersion fixed mouse splenocytes either stimulated with PHA (left panel) or untreated (right panel) using Goat Anti-Mouse CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1163) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
Proliferation Induced by CD40 Ligand/TNFSF5 and Neutralization by Mouse CD40 Ligand/TNFSF5 Antibody.
Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) induces proliferation in mouse splenic B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse CD40 Ligand/TNFSF5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1163). The ND50 is typically 0.08-0.4 µg/mL.


目次に戻る

Related Product & Information

Entrez Gene IDs959 (Human); 21947 (Mouse); 84349 (Rat)
BackgroundCD40 Ligand/TNFSF5
background_contentBackground:
CD40 Ligand/TNFSF5
CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid (aa) type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site (1, 2). CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes (3). Both membrane bound and soluble CD40L induce similar effects on B cells (3, 4). The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells, and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the soluble trimeric form of CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members (2). The genetic defect in the hyper-IgM syndrome is due to point mutations or deletions of the gene encoding the CD40L, which prevent CD40L from interacting with CD40 (5-7). CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into the cell cycle, isotype switching, Ig secretion, and memory generation (8, 9). CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation (10).


目次に戻る

Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Vaccination with a fusion protein that introduces HIV-1 gag antigen into a multitrimer CD40L construct results in enhanced CD8+ T cell responses and protection from viral challenge by vaccinia-gag.
    Authors: Gupta, Sachin, Termini, James M, Raffa, Francesc, Williams, Cindi-An, Kornbluth, Richard, Stone, Geoffrey
    J Virol, 2014;88(3):1492-501.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB

  2. The tumor necrosis factor family receptors RANK and CD40 cooperatively establish the thymic medullary microenvironment and self-tolerance.
    Authors: Akiyama T, Shimo Y, Yanai H, Qin J, Ohshima D, Maruyama Y, Asaumi Y, Kitazawa J, Takayanagi H, Penninger JM, Matsumoto M, Nitta T, Takahama Y, Inoue J
    Immunity, 2008;29(3):423-37.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

  3. CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals.
    Authors: Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA
    Vaccine, 2008;26(32):4062-72.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB

  4. Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific manner.
    Authors: Koguchi Y, Thauland TJ, Slifka MK, Parker DC
    Blood, 2007;110(7):2520-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

  5. Reduced pathology following infection with transgenic Leishmania major expressing murine CD40 ligand.
    Authors: Field AE, Wagage S, Conrad SM
    Infect. Immun., 2007;75(6):3140-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development



目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。