抗IL-18 Binding Protein-d抗体(Anti-IL-18 Binding Protein-d, Mouse, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：26765
IL-18 Binding Protein-dに対する抗体(Anti-IL-18 Binding Protein-d, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年06月26日 00時01分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-IL-18 Binding Protein-d, Mouse, Goat-Poly |
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1 | |||
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Anti-Mouse IL-18 BPd Affinity Purified Polyclonal Ab |
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0 | |||
[在庫・価格 ：2024年06月26日 00時01分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-18 Binding Protein-d, Mouse, Goat-Poly
文献数： 1
- 商品コード：AF122
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Mouse IL-18 BPd Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF122-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 BPdThr27-Ala191Accession # AAD17194 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL‑18 BPd in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse IL-18 BPc is observed and less than 2% cross-reactivity with recombinant human IL-18 BPa is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑18 BPd Fc Chimera (Catalog # 122-BP) |
| ELISA | This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse IL‑18 BPd Monoclonal Antibody (Catalog # MAB122). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-18 BPd DuoSet ELISA Kit (Catalog # DY122-05) for convenient development of a sandwich ELISA. | |
| Neutralization | Measured by its ability to neutralize IL‑18 BPd inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 2 µg/mL Recombinant Mouse IL‑18 BPd Fc Chimera, 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4, and 0.1 ng/mL Recombinant Mouse IL‑12. | |
| Neutralization | |
|---|---|
 | IL‑18 BPd Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Mouse IL‑18 BPd Antibody. Recombinant Mouse IL‑18 BPd Fc Chimera (Catalog # 122-BP) inhibits Recombinant Mouse IL‑18/IL‑1F4 induced IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Inhibition of Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) activity elicited by Recombinant Mouse IL‑18 BPd Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑18 BPd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF122). The ND50 is typically 10-30 µg/mL in the presence of Recombinant Mouse IL‑12 (0.1 ng/mL). |
| ELISA | |
 | Mouse IL‑18 BPd ELISA Standard Curve. Recombinant Mouse IL‑18 BPd protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑18 BPd Monoclonal Antibody (Catalog # MAB122) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑18 BPd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF122) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994). |
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Related Product & Information
| Long Name | Interleukin 18 Binding Protein Isoform d |
|---|---|
| Entrez Gene IDs | 10068 (Human); 16068 (Mouse) |
| Background | IL-18 BPd |
| background_content | Background: IL-18 BPd Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague.
PLoS Pathog, 2015;11(5):e1004921.
Species: Mouse
Sample Type: In Vivo
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