抗PDGF抗体(Anti-PDGF, Human, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26737

PDGFに対する抗体(Anti-PDGF, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-PDGF, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 4
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説明文
別名:PDGF
Genbank No: 5154
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Multi-species 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2025年06月06日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-PDGF, Human, Goat-Poly

文献数: 4

説明文 別名:PDGF
Genbank No: 5154
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Multi-species 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

LabelUnconjugated
ImmunogenHuman platelet-derived PDGF
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects PDGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize recombinant human (rh) PDGF‑AB, rhPDGF‑AA, rhPDGF‑BB, and natural porcine PDGF‑BB.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLHuman PDGF (Catalog # 120-HD)
NeutralizationMeasured by its ability to neutralize PDGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 3-5 µg/mL in the presence of 10 ng/mL Human PDGF.


Neutralization
Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody.
Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-PDGF Polyclonal Antibody (Catalog # AB-23-NA). The ND50 is typically 3-5 µg/mL.


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Related Product & Information

Long NamePlatelet-derived Growth Factor
Entrez Gene IDs5154 (Human)
BackgroundPDGF
background_contentBackground:
PDGF
PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
    Authors: Wong AP, Nili N, Strauss BH
    Cardiovasc. Res., 2005;65(3):702-10.
    Species: Rabbit
    Sample Type: Cell Culture Supernates
    Application: WB

  2. Differential role of platelet granular mediators in angiogenesis.
    Authors: Brill A, Elinav H, Varon D
    Cardiovasc. Res., 2004;63(2):226-35.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut



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