抗p38ポリクローナル抗体(Anti-p38) p38 antibody | StressMarq Biosciences

Multi-blot analysis of p38 in a cell lysate from 12 human cancer cell lines using a 1:1000 dilution of SPC-172.

p38 Antibody

Polyclonal

Anti-p38

Cell Signaling

CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A

N/A

Rabbit

N/A

A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH.

WB, IP, IHC

Human, Monkey, Mouse, Rat, Bovine, Rabbit, Pig, Canine, Hamster, Chicken, Sheep, Guinea pig

NP_001306.2

1433

Q16540

Detects a ~43kDa protein corresponding to the molecular mass of p38 on SDS PAGE immunoblots.

1:5000 (ECL) (WB), 1:250 (IP)

Rabbit Antiserum

Rabbit Antiserum

A 1:5000 dilution of SPC-172 was sufficient for detection of p38 in 20µg of HeLa cell lysate by ECL immunoblot analysis.

Research Background
The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

References
1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183.

2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38.

3. Han, J. et al. (1994) Science 265: 808-811.

4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948.

5. Deng et al. (2003) Cell. 115: 61-70.

6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899- 907.

7. Medicherla S. et al. (2006). J Pharmacol Exp Ther. 318(1): 99-107.