抗Phosphotyrosine抗体 | Anti-Phosphotyrosine antibody
掲載日情報：2018/10/03 現在Webページ番号：252161
StressMarq Biosciences社の抗Phosphotyrosine抗体(Anti-Phosphotyrosine antibody)です。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年04月16日 14時55分現在]
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Anti-Phosphotyrosine, Rabbit-Poly, HRP |
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[在庫・価格 ：2024年04月16日 14時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Phosphotyrosine, Rabbit-Poly, HRP
文献数： 0
- 商品コード：SPC-162F
- メーカー：STQ
- 包装：400μl
- 本製品は取扱中止になりました
| 説明文 | |||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Rabbit | |
| 交差性 | Species Independent | 適用 | ELISA,IC,IF,IHC,IP,Western Blot |
| 標識 | HRP | 性状 | Affinity Purified |
| 吸収処理 | クラス | ||
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
抗リン酸化アミノ酸抗体 |
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| 関連記事 | |||
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製品情報
 | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. |
 | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. |
 | Immunohistochemistry analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody conjugated to Biotin (SPC-161F-BI). Tissue: breast cancer. Species: Human. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161F-BI) at 1:100. |
Product Name
Phosphotyrosine Antibody
Clonality
Polyclonal
Description
Rabbit Anti-Phosphotyrosine Polyclonal
Research Areas
Cell Signaling, Phosphorylation, Post-translational Modifications
Alternative Names
Phospho-tyrosine Antibody
Host Species
Rabbit
Immunogen
Phosphotyrosine conjugated to KLH
Applications
WB, IHC, ICC/IF, IP, ELISA
Species Reactivity
Species Independent
Specificity
Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Purification
Peptide Affinity Purified
Storage Buffer
PBS pH7.0, 50% glycerol, 0.01% sodium azide
Certificate of Analysis
A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.
References
Scientific Background
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
References
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.
6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.
7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
References
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.
6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.
7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
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