抗Anti-Mouse CXCL1/KC MAb (Clone 1174A)抗体(Anti-Mouse CXCL1/KC MAb (Clone 1174A antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC‑1Asn29-Lys96Accession # P12850
Source	Recombinant Monoclonal Rabbit IgG Clone # 1174A
Purification	Protein A or G purified from cell culture supernatant
Specificity	Detects mouse CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	0.25-1 µg/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of CXCL1/GRO alpha /KC/CINC‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) treated with 1 μg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF‑ alpha Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

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Related Product & Information

Entrez Gene IDs	2919 (Human); 14825 (Mouse); 81503 (Rat)
Background	CXCL1/GRO alpha/KC/CINC-1
background_content	Background: CXCL1/GRO alpha/KC/CINC-1 KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet‑derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulant for CFU-GM.

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