抗Anti-Mouse CXCR5 Allophycocyanin MAb (Clone 614641)抗体(Anti-Mouse CXCR5 Allophycocyanin MAb (Clone 614641 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant mouse CXCR5Met1-Pro57Accession # Q04683
Source	Monoclonal Rat IgG2A Clone # 614641
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects recombinant mouse (rm) CXCR5 in direct ELISAs. In this format, no cross-reactivity with rmCXCR7 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/106 cells	See below

Flow Cytometry
	Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CXCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CXCR5 in Mouse T Follicular Helper Cells by Flow Cytometry. Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	643 (Human); 12145 (Mouse); 29363 (Rat)
Background	CXCR5
background_content	Background: CXCR5 CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

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Citations

1. Inhibition of increased circulating Tfh cell by anti-CD20 monoclonal antibody in patients with type 1 diabetes.
   Authors: Xu, Xinyu, Shi, Yun, Cai, Yun, Zhang, Qingqing, Yang, Fan, Chen, Heng, Gu, Yong, Zhang, Mei, Yu, Liping, Yang, Tao
   PLoS ONE, 2013;8(11):e79858.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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