抗Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22)抗体(Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22 antibody)

掲載日情報:2018/11/26 現在Webページ番号:231837

Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22)に対する抗体(Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22 )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22)
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説明文
別名:CD64
クローン:22
Genbank No: 2209
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保存条件 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 FITC
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年05月31日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human Fc gamma RI/CD64 Fluorescein MAb (Clone 22)

文献数: 0

説明文 別名:CD64
クローン:22
Genbank No: 2209
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 FCM
標識 FITC 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

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Product Details

Species ReactivityHuman
LabelFluorescein
ImmunogenIFN-y treated monocytes
SourceMonoclonal Mouse IgG1 Clone # 22
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects human Fc gamma RI/CD64 in direct ELISAs.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of Fc gamma RI/CD64 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 FITC-conjugated Monoclonal Antibody (Catalog # FAB12572F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundFc gamma RI/CD64
background_contentBackground:
Fc gamma RI/CD64
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors(~10‑8 ‑ 10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6 ‑ 10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associatedFc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up‑regulated during bacterial infections and sepsis.


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