抗CD27 [RM27-3E5]組換え抗体 | Anti-CD27 [RM27-3E5] recombinant antibody
掲載日情報:2019/10/24 現在Webページ番号:219194
Absolute Antibody社の抗CD27 [RM27-3E5]組換え抗体(リコンビナント抗体)( Anti-CD27 [RM27-3E5] recombinant antibody)です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2024年09月22日 00時00分現在]
詳細 | 商品名 |
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Anti-CD27, Mouse-Mono [RM27-3E5], IgG1-D265A, Purified Recombinant Antibody |
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Anti-CD27, Mouse-Mono [RM27-3E5], IgG1-D265A, Purified Recombinant Antibody |
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0 | |||
[在庫・価格 :2024年09月22日 00時00分現在]
Anti-CD27, Mouse-Mono [RM27-3E5], IgG1-D265A, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00895-1.32
- メーカー:ABA
- 包装:200μg
- 価格:¥148,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:TNFRS7,CD27 antigen,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7 クローン:RM27-3E5 |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | FCM,IP |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
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製品記事 | |||
関連記事 |
Anti-CD27, Mouse-Mono [RM27-3E5], IgG1-D265A, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00895-1.32-BT
- メーカー:ABA
- 包装:1mg
- 価格:ご照会ください
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:TNFRS7,CD27 antigen,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7 クローン:RM27-3E5 |
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---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | FCM,IP |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
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||
製品記事 | |||
関連記事 |
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製品情報
Description | Recombinant monoclonal antibody to CD27. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RM27-3E5. This antibody has a D265A mutation affecting Fc receptor engagement. This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
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Species and Isotype | Mouse IgG1-D265A, kappa |
Clone Number | RM27-3E5 |
UniProt Accession Number of Target Protein | P41272 |
Published Application(s) | Neutralisation; FC; IP |
Published Species Reactivity | Mouse |
Immunogen | This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas. |
Specificity | This antibody is specific for the extracellular domain of murine CD27, a TNF-receptor superfamily member. |
Application Notes | This antibody recognises murine CD27, as confirmed by flow-cytometry and immunoprecipitation analysis (Sakanishi & Yagita, 2010), a member of the TNF-receptor superfamily. The interaction between CD27 and its ligand CD70, which is recognised by anti-CD70 antibody TAN 1-7 (Ab00816), is crucial to regulation of the survival and differentiation of T cells, B cells and NK cells. As a result, RM27-3E5 modulates multiple immune cell types. RM27-3E5 does not deplete T cells, NKT or NK cells, but does enhance the proliferation of anti-CD3-stimulated splenic T cells (Sakanishi & Yagita, 2010). In murine models of melanoma, it promotes the persistence of tumour-specific CD8+ T cells within tumours, and reduces levels of PD-1 expression on CD8+ T cells (Roberts et al, 2010). Additionally, anti-CD27 reduces the proportion of FoxP3+ CD4+ T cells in tumours, and enhances the effector activity of IFNγ-secreting, tumour-infiltrating CD8+ T cells and NK cells (Roberts et al, 2010). Finally, anti-CD27 Ab treatment alters murine splenocyte cytokine production, significantly reducing IL-5 and IFN-γ synthesis, and increasing TNF-α production (Sumi et al, 2008). It also significantly suppresses total serum IgE levels, while significantly increasing IgG1 and IgG2a levels. These immune modulatory properties translate into potent anti-tumour effects in mice. Treatment of established murine melanoma with RM27-3E5 resulted in a significant reduction in lung metastases and subcutaneous tumours, through an IFNγ-dependent mechanism mediated by CD8+ T cells and NK cells (Roberts et al, 2010). In murine models of T cell lymphoma, the tumour-specific CTL response induced by RM27-3E5 promoted almost complete regression of tumours, regardless of CD27 expression on tumour cells (Sakanishi & Yagita, 2010). This antibody has been used to identify CD27-expressing cells through flow-cytometry (Koyanagi et al, 2012). |
Antibody First Published in | Sumi et al CD27 and CD70 do not play a critical role in the development of experimental allergic conjunctivitis in mice Immunology Letters, Volume 119, Issues 1–2, 15 August 2008, Pages 91-96 PMID:18579220 |
Note on publication | Describes the original generation of this antibody. |
Purification | Protein A affinity purified |
Supplied in | Standard size: PBS with 0.02% Proclin 300. Bulk size: PBS only. |
Storage Recommendation | Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C |
Concentration | See vial label. |
追加しました。
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