抗Lymphotoxin alpha [3F12.2D3]組換え抗体 | Anti-Lymphotoxin alpha [3F12.2D3] recombinant antibody
掲載日情報:2019/10/24 現在Webページ番号:218888
Absolute Antibody社の抗Lymphotoxin alpha [3F12.2D3]組換え抗体(リコンビナント抗体)( Anti-Lymphotoxin alpha [3F12.2D3] recombinant antibody)です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2026年04月06日 18時15分現在]
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Anti-Lymphotoxin alpha, Mouse IgG2b, Recombinant Monoclonal Antibody, clone 3F12.2D3 |
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Anti-Lymphotoxin alpha, Mouse IgG2b, Recombinant Monoclonal Antibody, clone 3F12.2D3 |
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[在庫・価格 :2026年04月06日 18時15分現在]
Anti-Lymphotoxin alpha, Mouse IgG2b, Recombinant Monoclonal Antibody, clone 3F12.2D3
文献数: 0
- 商品コード:Ab00788-3.0
- メーカー:ABA
- 包装:0.2mg
- 価格:¥143,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | 組換え体抗体。Azide Free。※カスタム対応可能:CONJUmab (Carrier Free, Concentrated, High Purity & Low Endotoxin) 抗体や標識抗体(Biotin修飾や蛍光標識など)の見積をご希望の場合は、弊社までお問い合わせください。バッファー組成:PBS with 0.02% Proclin 300. 別名:LT 3 chimeric 3F12,LT,,LT,Tumor necrosis factor beta,Tumor necrosis factor,Tumor necrosis factor-beta,tumor necrosis factor-,TNF beta,TNF-beta,TNF,TNF-,Lymphotoxin-alpha,Lymphotoxin alpha クローン:3F12.2D3 |
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| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising,SPR |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
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Anti-Lymphotoxin alpha, Mouse IgG2b, Recombinant Monoclonal Antibody, clone 3F12.2D3
文献数: 0
- 商品コード:Ab00788-3.0-BT
- メーカー:ABA
- 包装:1mg
- 価格:ご照会ください
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | 組換え体抗体。Azide Free。※カスタム対応可能:CONJUmab (Carrier Free, Concentrated, High Purity & Low Endotoxin) 抗体や標識抗体(Biotin修飾や蛍光標識など)の見積をご希望の場合は、弊社までお問い合わせください。バッファー組成:PBS only. 別名:LT 3 chimeric 3F12,LT,,LT,Tumor necrosis factor beta,Tumor necrosis factor,Tumor necrosis factor-beta,tumor necrosis factor-,TNF beta,TNF-beta,TNF,TNF-,Lymphotoxin-alpha,Lymphotoxin alpha クローン:3F12.2D3 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising,SPR |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | |||
追加しました。
製品情報
| Description | Recombinant monoclonal antibody to Lymphotoxin alpha. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F12.2D3. |
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| Species and Isotype | Mouse IgG2b, kappa |
| Clone Number | 3F12.2D3 |
| UniProt Accession Number of Target Protein | |
| Published Application(s) | neutralize; ELISA; FC;SPR |
| Published Species Reactivity | Human |
| Immunogen | 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTa expressed in E. coli - the hybridoma clone was selected for its ability to bind LTa3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody. |
| Specificity | 3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner. |
| Application Notes | The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding. |
| Antibody First Published in | Catellana et al. Resurrection of a Clinical Antibody: Template ProteoGenomic de novoProteomic Sequencing and Reverse Engineering of an Anti-Lymphotoxin Alpha Antibody Proteomics. 2011 Feb; 11(3): 395–405. PMID:21268269 |
| Note on publication | Describes the generation of anti-LT antibody and compares binding specificity and affinity of the chimeric antibody against the original antibody by BIACORE and ELISA. |
| Purification | Protein A affinity purified |
| Supplied in | Standard size: PBS with 0.02% Proclin 300. Bulk size: PBS only. |
| Storage Recommendation | Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C |
| Concentration | See vial label. |
追加しました。
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