抗Smad3(S423/S425) (5C5) Monoclonal抗体 | Anti-Smad3(S423/S425) (5C5) Monoclonal Antibody

• Bioss 社抗体製品の特長
• 価格表
• 使用例
• Background
• 製品情報

Bioss 社抗体製品の特長

• ウエスタンブロット、免疫組織化学（IHC-frozen、IHC-paraffin）、免疫蛍光（IF）、ELISAなどのアプリケーションに適した一次抗体がラインナップされています。
• 一次抗体はすべて、Protein AまたはProtein G精製されています。
• 幅広い研究分野に関連する抗体を取り揃えています。


※最新の適用、交差性情報の詳細は、下記製品情報項の「データシート」リンクをご参照ください。

目次に戻る

価格表

目次に戻る

使用例

	Lane 1: A431 cell lysates-untreated; Lane 2: A431 cell lysates-treated with TGF beta, probed with Smad3(S423/S425) (5C5 ) Monoclonal Antibody (bsm-52205R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
	A431 were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Smad3(S423/S425) (5C5) Monoclonal Antibody (bsm-52205R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
	Paraformaldehyde-fixed, paraffin embedded mouse skin section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Smad3(S423/S425) (5C5 ) Monoclonal Antibody (bsm-52205R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

目次に戻る

Background

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

目次に戻る

製品情報

name	Smad3(S423/S425) (5C5) Monoclonal Antibody
datasheet	最新のデータシートはこちらをご確認ください
Conjugation	Unconjugated
Gene ID	4088
Swiss Prot	P84022
Source	Synthetic peptide derived from human Smad3(S423/S425), around 400-450aa (phospho S423+S425).
Purification	Purified by Protein A.
Synonyms	Smad3 phospho Ser423; Smad3 phospho S423; p-Smad3 Ser423; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

目次に戻る