抗Estrogen Receptor alpha(S118) (3A7 ) Monoclonal抗体 | Anti-Estrogen Receptor alpha(S118) (3A7 ) Monoclonal Antibody

• Bioss 社抗体製品の特長
• 価格表
• 使用例
• Background
• 製品情報

Bioss 社抗体製品の特長

Bioss社は、米国ボストンに本拠地を置くメーカーで、約11,000種類の未標識一次抗体と、各抗体に対する12種類の標識製品をそろえています。そのラインナップは、約13万点にのぼります。

• ウエスタンブロット、免疫組織化学（IHC-frozen、IHC-paraffin）、免疫蛍光（IF）、ELISAなどのアプリケーションに適した一次抗体がラインナップされています。
• 一次抗体はすべて、Protein A精製後、さらに抗原ペプチドを用いてアフィニティ精製されています。
• 幅広い研究分野に関連する抗体を取り揃えています。


※最新の適用、交差性情報の詳細は、下記製品情報項の「データシート」リンクをご参照ください。

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価格表

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使用例

	MCF-7 cell lysates probed with Estrogen Receptor alpha(S118) (3A7 ) Monoclonal Antibody (bsm-52154R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
	Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Estrogen Receptor alpha(Ser118) (3A7) Monoclonal Antibody (bsm-52154R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
	HUVEC cells were stained with Estrogen Receptor alpha(S118) (3A7) Monoclonal Antibody (bsm-52154R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

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Background

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

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製品情報

name	Estrogen Receptor alpha(S118) (3A7 ) Monoclonal Antibody
datasheet	最新のデータシートはこちらをご確認ください
Conjugation	Unconjugated
Gene ID	2099
Swiss Prot	P03372
Source	Synthetic peptide derived from human Estrogen Receptor alpha(S118), around 180-220aa (phospho S209).
Purification	Purified by Protein A.
Synonyms	ER; ESR; Era; ESRA; ESTRR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1

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