抗HDAC2 (3B7) Monoclonal抗体 | Anti-HDAC2 (3B7) Monoclonal Antibody

• Bioss 社抗体製品の特長
• 価格表
• 使用例
• Background
• 製品情報

Bioss 社抗体製品の特長

• ウエスタンブロット、免疫組織化学（IHC-frozen、IHC-paraffin）、免疫蛍光（IF）、ELISAなどのアプリケーションに適した一次抗体がラインナップされています。
• 一次抗体はすべて、Protein AまたはProtein G精製されています。
• 幅広い研究分野に関連する抗体を取り揃えています。


※最新の適用、交差性情報の詳細は、下記製品情報項の「データシート」リンクをご参照ください。

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価格表

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使用例

	Lane 1: 293T Cell lysates; Lane 2: K562 lysates; probed with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
	SHG-44 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
	Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded mouse spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
	HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

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Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.

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製品情報

name	HDAC2 (3B7) Monoclonal Antibody
datasheet	最新のデータシートはこちらをご確認ください
Conjugation	Unconjugated
Gene ID	3066
Swiss Prot	Q92769
Source	Recombinant human HDAC2 protein, around C-terminal 150aa.
Purification	Purified by Protein A.
Synonyms	HD2; RPD3; YAF1; Histone deacetylase 2; HDAC2

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