抗FOXA1 (5F7) Monoclonal抗体 | Anti-FOXA1 (5F7) Monoclonal Antibody

• Bioss 社抗体製品の特長
• 価格表
• 使用例
• Background
• 製品情報

Bioss 社抗体製品の特長

• ウエスタンブロット、免疫組織化学（IHC-frozen、IHC-paraffin）、免疫蛍光（IF）、ELISAなどのアプリケーションに適した一次抗体がラインナップされています。
• 一次抗体はすべて、Protein AまたはProtein G精製されています。
• 幅広い研究分野に関連する抗体を取り揃えています。


※最新の適用、交差性情報の詳細は、下記製品情報項の「データシート」リンクをご参照ください。

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価格表

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使用例

	Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
	Paraformaldehyde-fixed, paraffin embedded mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
	MCF-7 cells were stained with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

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Background

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

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製品情報

name	FOXA1 (5F7) Monoclonal Antibody
datasheet	最新のデータシートはこちらをご確認ください
Conjugation	Unconjugated
Gene ID	3169
Swiss Prot	P55317
Source	Recombinant human FOXA1 protein, around 350-500aa.
Purification	Purified by Protein A.
Synonyms	HNF3A; TCF3A; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA1

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