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Recombinant 組換え体(リコンビナント)タンパク質 | Dkk-1, Human, Recombinant

掲載日情報：2018/02/08 現在Webページ番号：216350

アールアンドディー システムス /
R&D Systems, Inc.
[メーカー略称：RSD]

R&D Systems社製の高品質なRecombinantの組換え体タンパク質(Dkk-1, Human, Recombinant)です。
※本製品は研究用です。研究用以外には使用できません。

[在庫・価格 ：2024年05月21日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Dkk-1, Human, Recombinant
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 41
説明文
産生：Sf 21 (baculovirus)，純度：>95%, by SDS-PAGE with silver staining.，由来動物：Human，M.W.：25.8 kDa，Genbank：22943，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：dickkopf (Xenopus laevis) homolog 1
Genbank No： 22943
Protein Accession No： O94907
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Dkk-1, Human, Recombinant, Carrier-free
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
産生：Sf 21 (baculovirus)，純度：>95%, by SDS-PAGE with silver staining.，由来動物：Human，M.W.：25.8 kDa，Genbank：22943，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：dickkopf (Xenopus laevis) homolog 1
Genbank No： 22943
Protein Accession No： O94907
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保存条件 -20℃ 法規備考
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[在庫・価格 ：2024年05月21日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Dkk-1, Human, Recombinant

文献数： 41

説明文 産生：Sf 21 (baculovirus)，純度：>95%, by SDS-PAGE with silver staining.，由来動物：Human，M.W.：25.8 kDa，Genbank：22943，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：dickkopf (Xenopus laevis) homolog 1
Genbank No： 22943
Protein Accession No： O94907
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事
関連記事

Dkk-1, Human, Recombinant, Carrier-free

文献数： 0

説明文 産生：Sf 21 (baculovirus)，純度：>95%, by SDS-PAGE with silver staining.，由来動物：Human，M.W.：25.8 kDa，Genbank：22943，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：dickkopf (Xenopus laevis) homolog 1
Genbank No： 22943
Protein Accession No： O94907
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法規制等
保存条件 -20℃ 法規備考
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CF(Carrier-Free)とは？

R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。

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Product Details

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derivedThr32-His266
Accession #
O94907
Purity
>95%, by SDS-PAGE with silver staining.
Predicted Molecular Mass
25.8 kDa
SDS-PAGE
33-38 kDa, reducing conditions
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK) inhibits a constant dose of 500 ng/mL ofRecombinantHuman Wnt‑3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL. Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND50 for this effect is 0.03‑0.15 μg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Bioactivity
Recombinant Human Wnt‑3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog #5439‑DK) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED50 for this effect is 10‑60 ng/mL (orange circles).
Bioactivity2
Recombinant Human Dkk-1 (Catalog # 5439‑DK) inhibits Wnt-3a-induced alkaline phosphatase production by the MC3T3‑E1 mouse preosteoblast cell line. The ED50 for this effect is 0.03‑0.15 µg/mL.
SDS-PAGE
1 μg/lane of Recombinant Human Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 33-38 kDa. Multiple bands in gel are due to variable glycosylation.

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Related Product & Information

Entrez Gene IDs
22943 (Human); 13380 (Mouse)
Background
Dkk-1
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Autocrine WNT2 signaling in fibroblasts promotes colorectal cancer progression
Authors: N Kramer, J Schmöllerl, C Unger, H Nivarthi, A Rudisch, D Unterleuth, M Scherzer, A Riedl, M Artaker, I Crncec, D Lenhardt, T Schwarz, B Prieler, X Han, M Hengstschl, J Schüler, R Eferl, R Moriggl, W Sommergrub, H Dolznig
Oncogene, 2017;0(0):.
Species: N/A
Sample Type: Proteins
Application: Bioassay
Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor
Authors: ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim
J. Biol. Chem., 2017;0(0):.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
Human MSCs promotes colorectal cancer epithelial-mesenchymal transition and progression via CCL5/?-catenin/Slug pathway
Authors: K Chen, Q Liu, LL Tsang, Q Ye, HC Chan, Y Sun, X Jiang
Cell Death Dis, 2017;8(5):e2819.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Novel regulatory mechanisms for the SoxC transcriptional network required for visual pathway development
Authors: KC Chang, J Hertz, X Zhang, XL Jin, P Shaw, BA Derosa, JY Li, P Venugopala, DA Valenzuela, RD Patel, KR Russano, SA Alshamekh, C Sun, K Tenerelli, C Li, D Velmeshev, Y Cheng, TM Boyce, A Dreyfuss, MS Uddin, KJ Muller, DM Dykxhoorn, JL Goldberg
J. Neurosci., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway
Authors: Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen
Stem Cell Res Ther, 2017;8(1):98.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt7a Inhibits IL-1? Induced Catabolic Gene Expression and Prevents Articular Cartilage Damage in Experimental Osteoarthritis
Authors: AL Gibson, CK Hui Mingal, AT Foote, T Uchimura, M Zhang, L Zeng
Sci Rep, 2017;7(0):41823.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
WNT/?-Catenin signaling pathway regulates non-tumorigenesis of human embryonic stem cells co-cultured with human umbilical cord mesenchymal stem cells
Authors: YH Chang, TY Chu, DC Ding
Sci Rep, 2017;7(0):41913.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Experimental Study of the Biological Properties of Human Embryonic Stem Cell-Derived Retinal Progenitor Cells
Authors: J Shao, PY Zhou, GH Peng
Sci Rep, 2017;7(0):42363.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium
Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
COX-2 Induces Breast Cancer Stem Cells via EP4/PI3K/AKT/NOTCH/WNT Axis
Authors: Mousumi Majumder
Stem Cells, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015;6(0):6156.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Isolation and characterization of embryonic stem cell-derived cardiac Purkinje cells.
Authors: Maass K, Shekhar A, Lu J, Kang G, See F, Kim E, Delgado C, Shen S, Cohen L, Fishman G
Stem Cells, 2015;33(4):1102-12.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs).
Authors: Viale-Bouroncle S, Klingelhoffer C, Ettl T, Reichert T, Morsczeck C
Cell Signal, 2015;27(3):598-605.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Influence of Sex on Basal and Dickkopf-1 Regulated Gene Expression in the Bovine Morula.
Authors: Denicol A, Leao B, Dobbs K, Mingoti G, Hansen P
PLoS ONE, 2015;10(7):e0133587.
Species: Bovine
Sample Type: Whole Tissue
Application: Bioassay
Elevated levels of Dickkopf-1 are associated with beta-catenin accumulation and poor prognosis in patients with chondrosarcoma.
Authors: Chen C, Zhou H, Zhang X, Ma X, Liu Z, Liu X
PLoS ONE, 2014;9(8):e105414.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Inner ear hair cell-like cells from human embryonic stem cells.
Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
Stem Cells Dev, 2014;23(11):1275-84.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
PLoS ONE, 2014;9(8):e104937.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype.
Authors: Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, Halder J, Affatigato L, Mullins R, Stone E, Tucker B
Gene Ther, 2014;21(7):662-72.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
FGF16 promotes invasive behavior of SKOV-3 ovarian cancer cells through activation of mitogen-activated protein kinase (MAPK) signaling pathway.
Authors: Basu M, Mukhopadhyay S, Chatterjee U, Roy S
J Biol Chem, 2014;289(3):1415-28.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway.
Authors: Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, Guo L, Zhang Y, Chen G
PLoS ONE, 2014;9(5):e97283.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
J Biol Chem, 2013;288(36):26301-10.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
Cell Stem Cell, 2013;12(5):559-72.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
Authors: Tucker, Budd A, Mullins, Robert F, Streb, Luan M, Anfinson, Kristin, Eyestone, Mari E, Kaalberg, Emily, Riker, Megan J, Drack, Arlene V, Braun, Terry A, Stone, Edwin M
Elife, 2013;2(0):e00824.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.
Authors: Buchholz, David E, Pennington, Britney, Croze, Roxanne, Hinman, Cassidy, Coffey, Peter J, Clegg, Dennis O
Stem Cells Transl Med, 2013;2(5):384-93.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer.
Authors: Tahir S, Yang G, Goltsov A, Song K, Ren C, Wang J, Chang W, Thompson T
Cancer Res, 2013;73(6):1900-11.
Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
Authors: Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock B, Weller K, Wu H, Zhao S, Jin P, Dalton S
Stem Cell Reports, 2013;1(6):532-44.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Screening drug-induced arrhythmia [corrected] using human induced pluripotent stem cell-derived cardiomyocytes and low-impedance microelectrode arrays.
Authors: Navarrete E, Liang P, Lan F, Sanchez-Freire V, Simmons C, Gong T, Sharma A, Burridge P, Patlolla B, Lee A, Wu H, Beygui R, Wu S, Robbins R, Bers D, Wu J
Circulation, 2013;128(11):S3-13.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease-specific patterns of cardiotoxicity.
Authors: Liang, Ping, Lan, Feng, Lee, Andrew S, Gong, Tingyu, Sanchez-Freire, Veronica, Wang, Yongming, Diecke, Sebastia, Sallam, Karim, Knowles, Joshua W, Wang, Paul J, Nguyen, Patricia, Bers, Donald M, Robbins, Robert C, Wu, Joseph C
Circulation, 2013;127(16):1677-91.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Unraveling the signaling pathways promoting fibrosis in Dupuytren's disease reveals TNF as a therapeutic target.
Authors: Verjee L, Verhoekx J, Chan J, Krausgruber T, Nicolaidou V, Izadi D, Davidson D, Feldmann M, Midwood K, Nanchahal J
Proc Natl Acad Sci U S A, 2013;110(10):E928-37.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Dickkopf 1 promotes regression of hair follicles.
Authors: Kwack MH, Kim MK, Kim JC, Sung YK
J. Invest. Dermatol., 2012;132(6):1554-60.
Species: Mouse
Sample Type: In Vivo
Application: In Vivo
The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
J. Invest. Dermatol., 2011;131(3):644-54.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
PLoS ONE, 2011;6(1):e16366.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Neuroblastoma and bone metastases: clinical significance and prognostic value of Dickkopf 1 plasma levels.
Authors: Granchi D, Corrias MV, Garaventa A, Baglio SR, Cangemi G, Carlini B, Paolucci P, Giunti A, Baldini N
Bone, 2011;48(1):152-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
Cell, 2011;145(6):926-40.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
Authors: Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
Cell Res., 2011;21(4):579-87.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Efficient derivation of functional floor plate tissue from human embryonic stem cells.
Authors: Fasano CA, Chambers SM, Lee G, Tomishima MJ, Studer L
Cell Stem Cell, 2010;6(4):336-47.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells.
Authors: Cho SW, Yang JY, Sun HJ, Jung JY, Her SJ, Cho HY, Choi HJ, Kim SW, Kim SY, Shin CS
Bone, 2009;44(6):1069-77.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Dkk-3 is elevated in CSF and plasma of Alzheimer's disease patients.
Authors: Zenzmaier C, Marksteiner J, Kiefer A, Berger P, Humpel C
J. Neurochem., 2009;110(2):653-61.
Species: N/A
Sample Type: N/A
Application: IHC absorption control
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells.
Authors: Koch S, Capaldo CT, Samarin S, Nava P, Neumaier I, Skerra A, Sacks DB, Parkos CA, Nusrat A
Mol. Biol. Cell, 2009;20(22):4816-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay

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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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