HGF 組換え体(リコンビナント)タンパク質 | Recombinant Human HGF
掲載日情報：2018/02/07 現在Webページ番号：215997
R&D Systems社製の高品質なHGFの組換え体タンパク質(Recombinant Human HGF)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Recombinant Human HGF
文献数： 0
- 商品コード：294-HGN-01M
- メーカー：RSD
- 包装：1mg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
純度：>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.，由来動物：Human，M.W.：alpha chain: 53.7 kDa beta chain: 26 kDa，Genbank：3082，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：NS0 別名：deafness Genbank No： 3082 Protein Accession No： P14210 |
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| 保存条件 | -20℃ | 法規備考 | |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは？
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- Mouse myeloma cell line, NS0-derivedGln32-Ser728
- Accession #
- P14210
- Purity
- >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Predicted Molecular Mass
- alpha chain: 53.7 kDa beta chain: 26 kDa
- SDS-PAGE
- 65-75 kDa, non-reducing conditions60-65 kDa and 30-40 kDa, reducing conditions
- Activity
- Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1-0.5 ng/mL.The specific activity of recombinant human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
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Related Product & Information
- Entrez Gene IDs
- 3082 (Human); 15234 (Mouse); 403441 (Canine)
- Background
- HGF
- HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5) Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer
Authors: A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
Sci Rep, 2017;7(0):43736.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
Sci Rep, 2017;7(0):43736.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification.
Authors: Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, Oleksijew A, Boghaert E, Song M, Sokolova I, Pestova E, Anderson M, Pappano W, Ansell P, Bhathena A, Naumovski L, Corvaia N, Reilly E
BMC Cancer, 2016;16(1):105.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, Oleksijew A, Boghaert E, Song M, Sokolova I, Pestova E, Anderson M, Pappano W, Ansell P, Bhathena A, Naumovski L, Corvaia N, Reilly E
BMC Cancer, 2016;16(1):105.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion.
Authors: Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J
Oncogene, 2015;0(0):ePub.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J
Oncogene, 2015;0(0):ePub.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET.
Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
Clin Cancer Res, 2013;19(9):2381-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
Clin Cancer Res, 2013;19(9):2381-92.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
PLoS ONE, 2010;5(8):e12101.
Species: Human
Sample Type: Whole Cells
Application: Expansion/Differentiation
Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
PLoS ONE, 2010;5(8):e12101.
Species: Human
Sample Type: Whole Cells
Application: Expansion/Differentiation
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: N/A
Sample Type: N/A
Application: ELISA Standard
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: N/A
Sample Type: N/A
Application: ELISA Standard
Hepatocyte growth factor and its role in the pathogenesis of retinal detachment.
Authors: Jin M, Chen Y, He S, Ryan SJ, Hinton DR
Invest. Ophthalmol. Vis. Sci., 2004;45(1):323-9.
Species: Rabbit
Sample Type: In Vivo
Application: In Vivo
Authors: Jin M, Chen Y, He S, Ryan SJ, Hinton DR
Invest. Ophthalmol. Vis. Sci., 2004;45(1):323-9.
Species: Rabbit
Sample Type: In Vivo
Application: In Vivo
Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
Cancer Res., 2003;63(4):887-92.
Species: Human
Sample Type: Whole Cells
Application: Binding Assay
Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
Cancer Res., 2003;63(4):887-92.
Species: Human
Sample Type: Whole Cells
Application: Binding Assay
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