抗Human Cadherin-12 Alexa Fluor 647抗体(Anti-Human Cadherin-12 Alexa Fluor 647 antibody)
掲載日情報：2018/11/26 現在Webページ番号：207860
Human Cadherin-12 Alexa Fluor 647に対する抗体(Anti-Human Cadherin-12 Alexa Fluor 647 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月18日 00時00分現在]
| 詳細 | 商品名 |
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文献数 | ||
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Anti-Human Cadherin-12 Alexa Fluor 647 Antibody (Clone 343621) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Anti-Human Cadherin-12 Alexa Fluor 647 Antibody (Clone 343621)
文献数： 0
- 商品コード：FAB2240R-100UG
- メーカー：RSD
- 包装：100μg
- 価格：¥69,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Alexa Fluor 647 |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin-12Met1-Pro609Accession # P55289 |
| Source | Monoclonal Rat IgG1 Clone # 343621 |
| Specificity | Detects human Cadherin‑12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -13, -17, rhE-Cadherin, rhP-Cadherin, rhR-Cadherin, or rhVR-Cadherin is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 0.25-1 µg/106 cells | Human neural progenitor cells differentiated by growth factor withdrawal
Note: Since classic Cadherins can be protected from trypsin treatment in the presence of Ca2+, cells in monolayer cultures are harvested with 0.01% Trypsin in the presence of 1-5 mM CaCl2 at 37° C. Flow cytometry can be performed according to the standard procedures, except that all the cell staining and washing steps are performed in the presence of Ca2+ and Mg2+ (e.g. using FACS buffer: PBS containing 1 mM CaCl2, 1 mM MgCl2, 2% FBS and 0.02% sodium azide). |
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Related Product & Information
| Background | Cadherin-12 |
|---|---|
| background_content | Background: Cadherin-12 The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1).Cadherin‑12, also known as brain-cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium-dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin-12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6). |
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