Human Hydroxylated-HIF-1alpha (P402) ELISA Kit
掲載日情報:2017/06/21 現在Webページ番号:204170
RayBiotech社のHuman Hydroxylated-HIF-1alpha (P402) ELISA Kitです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年11月06日 14時15分現在]
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Human Hydroxylated-HIF-1alpha (P402) ELISA (96-Well Strip Microplate Kit) |
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Human Hydroxylated-HIF-1alpha (P402) ELISA (96-Well Strip Microplate Kit) |
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[在庫・価格 :2024年11月06日 14時15分現在]
Human Hydroxylated-HIF-1alpha (P402) ELISA (1 x 96-Well Strip Microplate Kit)
文献数: 0
- 商品コード:PTE-HIF1A-P402-1
- メーカー:RAY
- 包装:1kit
- 価格:¥124,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 種交差性:Human |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
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製品記事 | HIF(Hypoxia Inducible Factor、低酸素誘導因子)関連製品 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
Human Hydroxylated-HIF-1alpha (P402) ELISA (96-Well Strip Microplate Kit)
文献数: 0
- 商品コード:PTE-HIF1A-P402-2
- メーカー:RAY
- 包装:2kits
- 価格:¥221,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 種交差性:Human |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 | HIF(Hypoxia Inducible Factor、低酸素誘導因子)関連製品 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
Human Hydroxylated-HIF-1alpha (P402) ELISA (96-Well Strip Microplate Kit)
文献数: 0
- 商品コード:PTE-HIF1A-P402-5
- メーカー:RAY
- 包装:5kits
- 価格:¥488,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 種交差性:Human |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 | HIF(Hypoxia Inducible Factor、低酸素誘導因子)関連製品 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
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製品説明
■Introduction
RayBio® Hydroxylated-HIF-1alpha (Pro402) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining hydroxylated HIF-1alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human hydroxylated-HIF-1alpha. An anti-pan HIF-1alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HIF-1alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-Hydroxylated-HIF-1alpha (Pro402) antibody is used to detect hydroxylated HIF-1alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hydroxylated HIF-1alpha (Pro402) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
■Product Features
- Rapidly measure Hydroxylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
■Specificity
The antibody pair provided in this kit recognizes human Hydroxylated-HIF-1alpha hydroxylated at site Proline-402
■Target Protein Information
Synonyms: Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting protein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loop-helix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8)
Accession Number: Q16665
Gene Names: HIF1A BHLHE78 MOP1 PASD8
Gene ID: 3091
■Species Detected
Human
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Hydroxylated Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
■Typical Data
Positive Control
HeLa cells were treated with MG132. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
MG132 and DMOG Inhibition of HeLa Cell Line
HeLa cells were treated or untreated with MG132 and DMOG. Cell lysates were analyzed using this ELISA and Western Blot.
■Storage/Stability
Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at –20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.
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製品詳細
■ Antigen Information
Gene Symbol:
- HIF1A
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Pathway:
- Apoptosis
Species Detected:
- Human
Solid Support: 96-well Microplate
Design Principle:
- Sandwich-based
Quantitative/Semi-Quantitative: Semi-Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Cell Lysates
- Tissue Lysates
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