RBP-4, Rat, EIA Kit

RayBiotech社のRBP-4, Rat, EIA Kit です。
※本製品は研究用です。研究用以外には使用できません。

■Introduction

RBP4 (retinol binding protein-4) is an adipocyte-secreted molecule that is elevated in the serum before the development of frank diabetes. In obesity and type 2 diabetes, expression of the GLUT4 glucose transporter is decreased selectively in adipocytes. Adipose-specific Glut4 knockout mice show insulin resistance secondarily in muscle and liver. Recent studies have shown that serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes. RBP4 levels are normalized by rosiglitazone, an insulin-sensitizing drug. Transgenic overexpression of human RBP4 or injection of recombinant RBP4 in normal mice causes insulin resistance. Conversely, genetic deletion of RBP4 enhances insulin sensitivity. Fenretinide, a synthetic retinoid that increases urinary excretion of RBP4, normalizes serum RBP4 levels and improves insulin resistance and glucose intolerance in mice with obesity induced by a high-fat diet. Increasing serum RBP4 induces hepatic expression of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK) and impairs insulin signalling in muscle. Thus, RBP4 is an adipocyte-derived 'signal' that may contribute to the pathogenesis of type 2 diabetes. Lowering RBP4 could be a new strategy for treating type 2 diabetes. Thus, RBP4 appears to identify insulin resistance and associated cardiovascular risk factors in subjects with varied clinical presentations.

■Product Features

■Specificity

The capture antibody provided in this kit recognizes human, mouse and rat Retinol Binding Protein 4 (RBP4).

■Target Protein Information

Synonyms: Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP)

Accession Number: P04916

Gene Names: Rbp4

Gene ID: 25703

■Application Notes

Kit Components

Other Materials Required

Protocol Outline

■Storage/Stability

Standard, Biotinylated Retinol Binding Protein 4 peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

■ Antigen Information

Gene Symbol:

■ Product Specifications

Size: 1, 2, or 5 x 96-Well Strip Microplate Kit

■ Array/ELISA Format

Species Detected:

Solid Support: 96-well Microplate

Design Principle:

Quantitative/Semi-Quantitative: Quantitative

Method of Detection: Colorimetric

Compatible Sample Types: