Inhibin-B, Mouse, EIA Kit
掲載日情報：2017/06/21 現在Webページ番号：202063
RayBiotech社のInhibin-B, Mouse, EIA Kit です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 ：2026年04月06日 00時00分現在]
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Inhibin-B, Mouse, EIA Kit, RayBio (96-Well Strip Microplate Kit) |
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Mouse Inhibin-B EIA Kit |
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[在庫・価格 ：2026年04月06日 00時00分現在]
Inhibin-B, Mouse, EIA Kit, RayBio (96-Well Strip Microplate Kit)
文献数： 0
- 商品コード：EIAM-INB-1
- メーカー：RAY
- 包装：1kit
- 本製品は取扱中止になりました
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | |||
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Inhibin-B, Mouse, EIA Kit, RayBio (96-Well Strip Microplate Kit)
文献数： 0
- 商品コード：EIAM-INB-2
- メーカー：RAY
- 包装：2kits
- 本製品は取扱中止になりました
| 説明文 | |||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | |||
追加しました。
製品説明
■Introduction
Inhibin B belongs to TGF-beta superfamily, which members also include Inhibin A, TGF-betas (transforming growth factors), activins, BMPs (bone morphogenetic proteins). Among these factors, inhibins are most closely related to activins.
The activin and inhibin are both dimeric protein complexes in structure, and in each complex, the two monomers are linked to one another by a single disulfide bond. In addition, both complexes are derived from the same family of related genes and proteins but differ in their subunit composition. Inhibins are disulfide-linked heterodimers comprising an alpha-subunit and beta-subunit.Inhibin B is composed of one alpha subunit and one beta B subunit; Inhibin A is composed of one alpha subunit and one beta A subunit. By contrast, activins are homo- or hetero-dimers, forming activin A (2 beta A subunits), activin B (2 beta B subunits), or activin AB (1 beta A subunit and 1 beta B subunit).
Inhibins have played important roles in regulating reproductive health. Inhibins and activins generally play opposing roles as modulators of the reproductive axis and have multiple roles in the regulation of reproductive physiology in both females and males. Recently the emerging roles for inhibins in tumorigenesis have been reported. Inhibins has been reported as potential tumor suppressors in several different tissues, including ovary, prostate, endometrium and breasts, with loss of inhibin expression or loss of sensitivity linked to tumor initiation and progression as well as poor patient survival.
■Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
■Specificity
The capture antibody provided in this kit recognizes human, mouse and rat Inhibin-B.
■Target Protein Information
Synonyms: Inhibin B (INHB) (Inhibin alpha chain and Inhibin beta B chain (Activin beta-B chain))
Accession Number: Inhibin alpha chain = Q04997
Inhibin beta B chain = Q04999
Gene Names: Inhibin alpha chain = Inha
Inhibin beta B chain = Inhbb
Gene ID: Inhibin alpha chain = 16322
Inhibin beta B chain = 16324
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
■Storage/Stability
Standard, Biotinylated Inhibin B peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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製品詳細
■ Antigen Information
Gene Symbol:
- INHA
- INHBB
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Species Detected:
- Human
- Mouse
- Rat
Solid Support: 96-well Microplate
Design Principle:
- Competition-based
Quantitative/Semi-Quantitative: Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Serum
- Cell Culture Supernatants
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