GLP-1 EIA Kit for Serum, Plasma, Culture Supernatant and Cell Lysates
掲載日情報：2017/06/21 現在Webページ番号：202020
RayBiotech社のGLP-1 EIA Kit for Serum, Plasma, Culture Supernatant and Cell Lysatesです。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 ：2024年05月25日 08時35分現在]
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GLP-1 EIA Kit for Serum, Plasma, Culture Supernatant and Cell Lysates, RayBio (1×96 well strip plate) |
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Glucagon-like peptide-1, Human, EIA Kit, RayBio (96-Well Strip Microplate Kit) |
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1 | |||
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Human GLP-1 EIA Kit |
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1 | |||
[在庫・価格 ：2024年05月25日 08時35分現在]
GLP-1 EIA Kit for Serum, Plasma, Culture Supernatant and Cell Lysates, RayBio (1×96 well strip plate)
文献数： 1
- 商品コード：EIA-GLP1-1
- メーカー：RAY
- 包装：1kit
- 価格：¥90,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human, Mouse, Rat，感度：1.17 pg, ml，測定範囲：0.1-1,000 pg, ml，検出方法：呈色，測定波長：450 nm，アッセイ法：Competition-based，アッセイ数：96 well，測定試料：細胞培養上清, 血清 別名：GCG |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | |||
Glucagon-like peptide-1, Human, EIA Kit, RayBio (96-Well Strip Microplate Kit)
文献数： 1
- 商品コード：EIA-GLP1-2
- メーカー：RAY
- 包装：2kits
- 価格：¥161,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human, Mouse, Rat，測定試料：細胞培養上清, 血清，アッセイ法：Competition-based，検出方法：呈色，感度：1.17 pg, ml，測定範囲：0.1-1,000 pg, ml 別名：GCG |
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|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
|
||
| 製品記事 | |||
| 関連記事 | |||
Human GLP-1 EIA Kit
文献数： 1
- 商品コード：EIA-GLP1-5
- メーカー：RAY
- 包装：5kits
- 価格：¥357,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human, Mouse, Rat，アッセイ法：Competition-based，検出方法：呈色，測定試料：細胞培養上清, 血清，感度：1.17 pg, ml，測定範囲：0.1-1,000 pg, ml 別名：GCG |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
|
||
| 製品記事 | |||
| 関連記事 | |||
追加しました。
製品説明
■Introduction
Glucagon-like peptide-1 (GLP-1) is a 31 amino acid peptide hormone derived from selective cleavage of the proglucagon gene. It is mainly produced from enteroendocrine L-cells in GI tract. The other cleavage products derived from proglucagon genes are glucagon, GLP-2 and other small fragment peptides including Glicentin, Oxyntomodulin and two intervening peptides (IP-1 and IP-2). Except for glucagon cleaved in alpha cells of the pancreas, all other cleaved peptides occurred in enteroendocrine L cells of intestine.
GLP-1 has shown important roles in regulating glucose metabolic functions in humans. There are studies showing that GLP-1 is a potent anti-hyperglycemic hormone inducing glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. This dual control of insulin and glucagon has the benefit that the plasma glucose concentration is kept in the normal fasting range to avoid hypoglycemia caused by overstimulation of insulin. In addition, GLP-1 was reported to restore the glucose sensitivity of pancreatic beta cells, probably via the upregualtion of GLUT2 and glucokinase. GLP-1 was reported to inhibit pancreatic beta-cell apoptosis and stimulate the proliferation and differentiation of insulin-secreting beta-cells.
GLP-1 has shown potential clinical application as a biomarker and treatment option for Diabetes Mellitus, which is based on the following physiological functions mediated by GLP-1.
- Regulating insulin secretion by increasing insulin secretion from the pancreas in a glucose-dependent manner and increasing insulin-sensitivity in both alpha and beta cells
- Regulating glucagon secretion by decreasing glucagon secretion from the pancreas by engagement of a specific G protein-coupled receptor.
- Regulating food intake by inhibiting acid secretion and gastric emptying in the stomach and decreasing food intake by increasing satiety in brain.
■Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
■Specificity
The capture antibody in this kit recognizes human, mouse and rat GLP1 (Glucagon-like Peptide 1), a cleavage product of GCG (Glucacon).
■Target Protein Information
Synonyms: Glucagon [Cleaved into: Glucagon-like peptide 1 (GLP-1) (Incretin hormone)]
Accession Number: P01275 (PRO_0000011258)
Gene Names: GCG
Gene ID: 2641
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
■Storage/Stability
Standard, Biotinylated GLP-1 peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
追加しました。
製品詳細
■ Antigen Information
Gene Symbol:
- GCG
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Species Detected:
- Human
- Mouse
- Rat
Solid Support: 96-well Microplate
Design Principle:
- Competition-based
Quantitative/Semi-Quantitative: Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Serum
- Cell Culture Supernatants
追加しました。
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