抗Human VSIG2抗体(Anti-Human VSIG2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VSIG2Val24-Ala243Accession # Q96IQ7
Source	Monoclonal Mouse IgG2B Clone # 541527
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VSIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VSIG1, 3, 4, or recombinant mouse VSIG2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below

Western Blot

Detection of Human VSIG2 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG2 Monoclonal Antibody (Catalog # MAB5177) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VSIG2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	V-Set and Immunoglobulin Domain Containing 2
Background	VSIG2
background_content	Background: VSIG2 VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Human VSIG2 precursor is 327  amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 24‑243) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 24‑137) and one C2-type Ig-like domain (aa 144‑233). One potential splice variant exists that shows a deletion of aa 285‑327. Over aa 24‑243, human VSIG2 is 85% aa identical to mouse VSIG2.

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