抗Mouse TLR11 Phycoerythrin抗体(Anti-Mouse TLR11 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant mouse TLR11Thr209-Gln325Accession # Q6R5P0
Source	Monoclonal Rat IgG2B Clone # 786404
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse TLR11 in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 PE‑conjugated Monoclonal Antibody (Catalog # IC7640P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Toll-like Receptor 11
Background	TLR11
background_content	Background: TLR11 TLR11 is a 110-120 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor family of proteins that is primarily expressed in intestinal and bladder epithelial cells, dendritic cells, macrophages, astrocytes, neurons and microglia. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats, a 21 aa transmembrane (TM) domain, and a 184 aa cytoplasmic region with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER) where it heterodimerizes with TLR12, and interacts with the 12-TM multispan ER protein UNC93B1, that serves as chaperone and co-factor for TLR11 activity. The receptor recognizes flagelin and profilin from Toxoplasma gondii, plus components of E.coli, S.typhimurium, and promotes IL-12 production by dendritic cells.

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