抗Myosin抗体(Anti-Myosin, Heavy Chain, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	Chicken pectoralis-derived Myosin
Source	Monoclonal Mouse IgG2B Clone # MF20
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of Myosin Heavy Chain in C2C12 Mouse Cell Line by Flow Cytometry. Three-day differentiated C2C12 mouse myoblast cell line was stained with Mouse Anti-Human Myosin Heavy Chain Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC4470F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

Myosin Heavy Chain

background_content

Background: Myosin Heavy Chain Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

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Citations

1. APOBEC2 negatively regulates myoblast differentiation in muscle regeneration
   Authors: H Ohtsubo, Y Sato, T Suzuki, W Mizunoya, M Nakamura, R Tatsumi, Y Ikeuchi
   Int. J. Biochem. Cell Biol, 2017;85(0):91-101.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle
   Authors: I Toral-Ojed, G Aldanondo, J Lasa-Elgar, H Lasa-Ferná, R Fernández-, A López de M, A Vallejo-Il
   Expert Rev Mol Med, 2016;18(0):e7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen

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