抗Human Flt-3/Flk-2 Alexa Fluor 700抗体(Anti-Human Flt-3/Flk-2 Alexa Fluor 700 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Alexa Fluor 700
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Flt‑3/Flk‑2Asn27-Asn541Accession # AAA18947
Source	Monoclonal Mouse IgG1 Clone # 66903
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detect human Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Flt-3, recombinant human (rh) SCF R, rhPDGF R alpha, or rhPDGF R beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below

Flow Cytometry
	Detection of Flt‑3/Flk‑2 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human Flt‑3/Flk‑2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB812N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	fms-like Tyrosine Kinase 3
Background	Flt-3/Flk-2
background_content	Background: Flt-3/Flk-2 The Flt-3 (fms-Like Tyrosine kinase) Receptor, also known as Flk-2 (Fetal Liver Kinase) and Stk-1(Stem cell Tyrosine Kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

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