抗Human CD7 Allophycocyanin抗体(Anti-Human CD7 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Human embryonic kidney cell line HEK293-derived human CD7Ala26-Pro180Accession # P09564
Source	Monoclonal Mouse IgG2B Clone # 848438
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD7 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of CD7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD7 APC‑conjugated Monoclonal Antibody (Catalog # FAB7579A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	924 (Human); 12516 (Mouse); 303747 (Rat)
Background	CD7
background_content	Background: CD7 CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34+ myeloid and lymphoid progenitor cells, memory CLA- CD45RA+ T cells, and CD56+ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

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