抗Mouse CD79B Phycoerythrin抗体(Anti-Mouse CD79B Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant mouse CD79BMet29-Asp158Accession # P15530
Source	Monoclonal Rat IgG2A Clone # 735451
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CD79B in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of CD79B in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD79B PE‑conjugated Monoclonal Antibody (Catalog # FAB7326P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	974 (Human); 15985 (Mouse); 171055 (Rat)
Background	CD79B
background_content	Background: CD79B CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta -chain) is a 37-39 kDa member of the Ig-Superfamily. It is expressed on B cells and forms a covalent heterodimer with CD79A. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. CD79B is also required for formation of pre-B cells during B cell development. Mature mouse CD79B is a 203 amino acid (aa) type I transmembrane glycoprotein (aa 26-228). It contains an extracellular region with one V-type Ig-like domain (aa 41-132) and an ITAM‑containing cytoplasmic domain (aa 181-228). CD79B may migrate as two bands in SDS-PAGE. One defines the standard 37 kDa form, while a second represents one of two possible isoforms, the first of which is an underglycosylated full-length CD79B, and the second of which is an alternative splice form that likely lacks the C‑terminal 32 amino acids. Mouse CD79A and CD79B share only 24% aa identity. Over aa 29-158, mouse CD79B shares 54% and 78% aa identity with human and rat CD79B, respectively.

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