抗Mouse FCRL1/FcRH1 Allophycocyanin抗体(Anti-Mouse FCRL1/FcRH1 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse FCRL1/FcRH1Ala17-Ser219Accession # Q8R4Y0
Source	Monoclonal Rat IgG1 Clone # 745916
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse FCRL1/FcRH1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FCRL1/FcRH1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of FCRL1/FcRH1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse FCRL1/FcRH1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7286A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Fc Receptor-like 1
Background	FCRL1/FcRH1
background_content	Background: FCRL1/FcRH1 FCRL1 (Fc Receptor-Like Protein 1), also known as CD307a, FcRH1 and IFGP1, is a 36 kDa (predicted) member of the Ig Superfamily. In mouse, it is found on mature follicular and marginal B cells, splenic T cells and NK cells. It would appear that mouse FCRL1 serves as an inhibiting coreceptor, in as much as it contains a series of cytoplasmic ITIM motifs. Mature mouse FCRL1 is a 327 amino acid (aa) type I transmembrane protein (aa 17-343). It contains a 203 aa extracellular region (aa 17‑219) that shows two C2-type Ig-like domains (aa 17-200), and a 103 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 206-248 (potentially soluble), a second contains a six aa substitution for aa 319-343, a third possesses a three aa substitution for aa 209-343, and a fourth shows a 21 aa substitution for Ala11. Over aa 17-219, mouse FCRL1 shares 83% aa sequence identity with rat FCRL1. Over the same region, it shares 63% aa sequence identity with human FCRL1 when a third Ig-like domain that is present in human is excluded from the analysis.

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