抗Mouse Semaphorin 4F Allophycocyanin抗体(Anti-Mouse Semaphorin 4F Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4F Arg41-Gly667Accession # Q9Z123
Source	Monoclonal Rat IgG2A Clone # 780225
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Semaphorin 4 in direct ELISAs.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of Semaphorin 4F in Mouse Cortical Stem Cells and bEnd.3 Mouse Cell Line by Flow Cytometry. Mouse cortical stem cells (A) and bEnd.3 mouse endothelioma cell line (B) were stained with Rat Anti-Mouse Semaphorin 4F APC‑conjugated Monoclonal Antibody (Catalog # FAB7200A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Entrez Gene IDs	10505 (Human); 20355 (Mouse); 29745 (Rat)
Background	Semaphorin 4F
background_content	Background: Semaphorin 4F Semaphorin 4F (Sema4F), also known as Sema W, is a 90-100 kDa class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa sequence identity with human and rat Sema4F, respectively.

目次に戻る