抗Human CD1a Alexa Fluor 700抗体(Anti-Human CD1a Alexa Fluor 700 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Alexa Fluor 700
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CD1aAsp19-Val300 (predicted)Accession # P06126
Source	Monoclonal Mouse IgG1 Clone # 703217
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD1a in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD1d is observed and no cross-reactivity with with rhCD1b or rhCD1e is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below

Flow Cytometry
	Detection of CD1a in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1a Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB7076N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	T Cell Surface Glycoprotein CD1a
Background	CD1a
background_content	Background: CD1a CD1a (also known as Leu6) ia a 48-50 kDa member of the five gene CD1 family of molecules. It is a type I transmembrane glycoprotein that is 311 amino acids (aa) in length. It contains three " alpha -domains" in its extracellular region (aa 17-300), and terminates with a short six aa segment. Although CD1a orthologs are found in multiple mammalian species, it does not appear to exist in rodent. Notably, not all humans possess a functional CD1a gene. CD1a serves as an MHC Class I type molecule that presents lipids to alpha beta TCRs on T cells. This means it exists as a heterodimer with beta 2-microglobulin on the cell surface. CD1a is unique in that it can present nonhydrophilic lipids to T cells; that is, there is no need for a polar "head" on one end of the lipid to interact with the polar residues of the TCR protein. Other cell membrane components suggested to associate with CD1a include CD74 and CD9, two molecules that may regulate cycling of CD1a. Cells that are known to express CD1a are restricted in number, and include cortical thymocytes, Langerhans cells, dermal dendritic cells (DC), and monocyte-derived DC. It has been suggested that type I mono-DCs, or those that secrete IL-12 and promote Th1 formation, are uniquely CD1a+. At this time, this appears to be too restrictive an assumption, and that the presence or absence of CD1a is only generally reflective of function.

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