抗Human 5'-Nucleotidase/CD73 Phycoerythrin抗体(Anti-Human 5'-Nucleotidase/CD73 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73Trp27-Ile511Accession # P21589
Source	Monoclonal Mouse IgG2B Clone # 606112
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'‑Nucleotidase/CD73 PE‑conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	4907 (Human); 23959 (Mouse); 58813 (Rat)
Background	5'-Nucleotidase/CD73
background_content	Background: 5'-Nucleotidase/CD73 5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.

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Citations

1. Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells
   Authors: SJ Li, Y Luo, LM Zhang, W Yang, GG Zhang
   Mol Med Rep, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors
   Authors: Christopher J Hewitt
   Biotechnol J, 2016;11(4):473-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
   Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
   Stem Cells Dev, 2014;23(5):541-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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