抗Human Integrin alpha 1/CD49a Alexa Fluor 488 Aff Pur PAb抗体(Anti-Human Integrin alpha 1/CD49a Alexa Fluor 488 Aff Pur PAb antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Alexa Fluor 488
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 1/CD49aPhe29-Pro1141Accession # P56199
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Integrin  alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin  alpha 2, rhIntegrin  alpha 11, recombinant mouse (rm) Integrin  alpha 2, and rmIntegrin  alpha 11 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below

Flow Cytometry

Detection of Integrin  alpha 1/CD49a in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin  alpha 1/CD49a Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5676G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Entrez Gene IDs	3672 (Human); 109700 (Mouse); 25118 (Rat)
Background	Integrin alpha 1/CD49a
background_content	Background: Integrin alpha 1/CD49a Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin  alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin  alpha 1 shares 88% aa identity with mouse Integrin  alpha 1.

目次に戻る