抗F4/80抗体(Anti-F4/80, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1Gln28-Gly931Accession # Q61549
Source	Monoclonal Rat IgG2A Clone # 521204
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse F4/80/EMR1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	13733 (Mouse); 316137 (Rat)
Background	F4/80
background_content	Background: F4/80 F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta + proinflammatory macrophages.

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Citations

1. IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
   Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
   J Immunol, 2015;194(8):3861-72.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection.
   Authors: Fermino M, Dias F, Lopes C, Souza M, Cruz A, Liu F, Chammas R, Roque-Barreira M, Rabinovich G, Bernardes E
   Eur J Immunol, 2013;43(7):1806-17.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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