抗PIR-B抗体(Anti-PIR-B, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse PIR-BGly24-Gly635Accession # AAH26937
Source	Monoclonal Rat IgG2A Clone # 326414
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse PIR‑B PE‑conjugated Monoclonal Antibody (Catalog # FAB2754P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Paired-Ig-like Receptor B
Background	PIR-B
background_content	Background: PIR-B Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

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Citations

1. CAMKs support development of acute myeloid leukemia
   Authors: X Kang, C Cui, C Wang, G Wu, H Chen, Z Lu, X Chen, L Wang, J Huang, H Geng, M Zhao, Z Chen, M Müschen, HY Wang, CC Zhang
   J Hematol Oncol, 2018;11(1):30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development.
   Authors: Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC
   Nature, 2012;485(7400):656-60.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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