抗Mouse IFN-gamma R1 Phycoerythrin抗体(Anti-Mouse IFN-gamma R1 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Recombinant mouse IFN‑ gamma R1/CD119Extracellular domain
Source	Monoclonal Hamster IgG1 Clone # 2E2.4
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma  R1, rhIFN-gamma R2, or rhIL-10 R beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection ofIFN‑ gamma  R1/CD119 in Mouse Splenocytes by FlowCytometry. CD11b+ mouse splenocyteswere stained with Hamster Anti-Mouse IFN‑ gamma  R1/CD119PE‑conjugated Monoclonal Antibody (Catalog # FAB1026P, filledhistogram) or isotype control (open histogram). View our protocol for StainingMembrane-associated Proteins.

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Related Product & Information

Long Name	Interferon gamma Receptor 1
Background	IFN-gamma R1/CD119
background_content	Background: IFN-gamma R1/CD119 The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall amino acid (aa) sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is both necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Mouse IFN-gamma  R1 cDNA encodes a 477 aa residue protein with a 22 aa signal peptide, a 231 aa extracellular domain, a 24 aa transmembrane segment, and a 200 aa intracellular domain. Human and mouse IFN-gamma  R1 share 50% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma  R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

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Citations

1. Important scaffold function of the Janus kinase 2 uncovered by a novel mouse model harboring a Jak2 activation-loop mutation.
   Authors: Keil E, Finkenstadt D, Wufka C, Trilling M, Liebfried P, Strobl B, Muller M, Pfeffer K
   Blood, 2014;123(4):520-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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