抗Human/Mouse/Rat gp96/HSP90B1抗体(Anti-Human/Mouse/Rat gp96/HSP90B1 antibody)
掲載日情報：2018/11/26 現在Webページ番号：197239
Human/Mouse/Rat gp96/HSP90B1に対する抗体(Anti-Human/Mouse/Rat gp96/HSP90B1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
カートに商品を
追加しました。
追加しました。
価格
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-Human/Mouse/Rat gp96/HSP90B1 Affinity Purified Polyclonal Ab |
|
1 | |||
|
Anti-Human/Mouse/Rat gp96/HSP90B1 Affinity Purified Polyclonal Ab |
|
1 | |||
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human/Mouse/Rat gp96/HSP90B1 Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF7606
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human/Mouse/Rat gp96/HSP90B1 Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF7606-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
カートに商品を
追加しました。
追加しました。
Product Details
| Species Reactivity | Human, Mouse, Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human gp96/HSP90B1 Arg503-Arg660Accession # P14625 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human, mouse, and rat gp96/HSP90B1 in direct ELISAs and Western blots. |
カートに商品を
追加しました。
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| Immunohistochemistry | 1-15 µg/mL | See below |
| Knockout Validated | Gp96/HSP90B1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in gp96/HSP90B1 knockout HEK293T cell line. | |
| Western Blot | |
|---|---|
 | Detection of Human, Mouse, and Rat gp96/HSP90B1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat gp96/HSP90B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7606) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gp96/HSP90B1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry | |
 | gp96/HSP90B1 in Human Mesothelioma Tissue. gp96/HSP90B1 was detected in immersion fixed paraffin-embedded sections of human mesothelioma tissue using Sheep Anti-Human/Mouse/Rat gp96/HSP90B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7606) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Knockout Validated | |
 | Western Blot Shows Human gp96/HSP90B1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and gp96/HSP90B1 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat gp96/HSP90B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7606) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gp96/HSP90B1 at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
カートに商品を
追加しました。
追加しました。
Related Product & Information
| Long Name | Heat Shock Protein 90 beta 1 |
|---|---|
| Background | gp96/HSP90B1 |
| background_content | Background: gp96/HSP90B1 Glycoprotein 96 (gp96; also endoplasmin, GRP-94, TRA1 and HSP90B1) is a 94-100 kDa member of the HSP 90 family of proteins. Gp96 is a ubiquitously-expressed, ER resident protein, and is found in a preformed complex with BiP, CaBP1 and UDP-glucosyltransferase. This is a chaperone complex that binds unfolded protein substrates. When folded properly, the substrate is forwarded to calnexin-containing chaperone complexes that promote its maturation. Gp96 clients are restricted, and include disulfide-bonded integrins, TLRs, LDLR and CD180. Within the complex, gp96 exists as a disulfide-linked homodimer that may form higher-order oligomers. Gp96 also appears on the cell surface, and may serve as a receptor for bacteria. Mature human gp96 is a 782 amino acid (aa) membrane-associated protein (aa 22‑803). It is not a transmembrane protein, but utilizes an ER retention signal (aa 800-803) to interact with the ER membrane. The molecule possesses a HATPase‑C like region (aa 98-219), plus multiple ATP binding and two utilized phosphorylation sites. Over aa 503‑660, human gp96 shares 98% aa sequence identity with mouse gp96. |
カートに商品を
追加しました。
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。