抗Human Neutrophil Elastase/ELA2抗体(Anti-Human Neutrophil Elastase/ELA2 antibody)
掲載日情報：2018/11/26 現在Webページ番号：196708
Human Neutrophil Elastase/ELA2に対する抗体(Anti-Human Neutrophil Elastase/ELA2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2026年04月23日 10時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human Neutrophil Elastase/ELA2 MAb (Clone 950317) |
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2 | |||
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Anti-Human Neutrophil Elastase/ELA2 MAb (Clone 950317) |
|
2 | |||
[在庫・価格 ：2026年04月23日 10時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Neutrophil Elastase/ELA2 MAb (Clone 950317)
文献数： 2
- 商品コード：MAB91671-100
- メーカー：RSD
- 包装：100μg
- 価格：¥79,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human Neutrophil Elastase/ELA2 MAb (Clone 950317)
文献数： 2
- 商品コード：MAB91671-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human ELA2Met1-Asn252Accession # P08246 |
| Source | Monoclonal Mouse IgG1 Clone # 950317 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human ELA2 in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | See below |
| Immunohistochemistry | 3-25 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 5-25 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Western Blot | |
|---|---|
 | Detection of Human Neutrophil Elastase/ELA2 by Western Blot. Western blot shows lysates of human bone marrow and human lung tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Neutrophil Elastase/ELA2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Neutrophil Elastase/ELA2 in THP‑1 Human Cell Line. Neutrophil Elastase/ELA2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of Neutrophil Elastase/ELA2 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line treated with 3 μM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
| Immunohistochemistry | |
 | Neutrophil Elastase/ELA2 in Human Lymphoma. Neutrophil Elastase/ELA2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Related Product & Information
| Background | Neutrophil Elastase/ELA2 |
|---|---|
| background_content | Background: Neutrophil Elastase/ELA2 Neutrophil Elastase (ELA2, ELANE), also known as HNE, is a chymotrypsin family serine protease that plays a key role in pathogen clearance (1-3). It is expressed by promyelocytes and stored in the intracellular azurophilic granules of polymorphonuclear leukocytes (PMN) (4). These granules fuse with phagosomes, enabling Neutrophil Elastase to participate in the digestion and killing of endocytosed microbes. The enzyme is released by activated neutrophils at sites of inflammation, and it can remain associated with the cell surface or function as a component of neutrophil extracellular nets (NETs) which trap and kill microbial pathogens (5, 6). It also can degrade multiple extracellular matrix proteins including Elastin and Fibronectin (5). In the lung, this activity contributes to pathology in emphysema, cystic fibrosis, and adult respiratory distress syndrome (ARDS) (1). Neutrophil Elastase can be inhibited by Serpin A1/alpha 1-Antitrypsin, SLPI, Serpin B1, andTrappin-2/Elafin (7-11). Its activity in the lung is increased by exposure to tobacco smoke which inactivates Serpin A1 through methionine oxidation (12). Mature human Neutrophil Elastase shares 73% amino acid sequence identity with mouse and rat Neutrophil Elastase (13, 14). Multiple mutations in the human ELANE gene are causative of severe congenital and cyclic neutropenias (15). |
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